Monitoring the modulation of cellular pathway activity has been recognized as a highly efficient method of determining efficacy and selectivity of drug candidates. By specifically profiling compounds in stress- and apoptosis pathways, these early indicators of toxicity may identify adverse drug effects at an earlier stage in the drug development process. This early elimination of potentially cytotoxic chemical entities will lower the increasingly high costs for development of new drugs.
Zeptosens offers two assay kits that allow the assessment of compounds on apoptosis as well as stress related signaling events as part of their protein profiling services :
Apoptosis Pathway | Stress-Induced Signaling Pathway
| | cleaved Caspase3 (Asp175) | P-c-Jun (Ser63) | | Bcl-2 | P-c-Jun (Ser73)
| | P-Bad (Ser112) | P-p38MAPK (Thr180, Tyr182)
if both and single P-sites phosphorylated | | P-Bad (Ser 136) | P-p38MAPK (Thr180, Tyr182)
only if both sites phosphorylated | | IκBα | P-MAPKAPK-2 (Thr334)
| | P-IκBα (Ser32) | P-MSK1 (Ser376)
| | p53 | P-CREB (Ser133)
| | P-p53 (Ser15) | P-p53 (Ser6) | Akt
| P-p53 (Ser15) | P-Akt (Ser473)
| P-p53 (Ser20) | | PKA | P-ATF-2 (Thr71) | P-PKA (Ser96)
| P-SAPK/JNK (Thr183, Tyr185) |
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Additional kits for other cellular pathway activities are expected soon.
Take advantage of our extensive experience in protein profiling applications to augment your research efforts in a cost-effective manner.
General references:
1: Proteomics - Clinical Applications: Tracing pathway activities with kinase inhibitors and reverse phase protein arrays
2: European Pharmaceutical Review: Antibody-based proteomics to study cellular signaling networks
Please contact Zeptosens for more information on indicative toxicology studies in your specific field of interest. Alternatively, Zeptosens enables you to run multiplexed indicative toxicology studies also in house.
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