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Reverse protein arrays require spotting of complex samples (derived from cells, tissues or body fluids) with precise deposition in sub-nanoliter volumes onto the chip surface for further probing with antibodies. For this part of the reverse array process, Zeptosens recommends non-contact inkjet spotters for superior results. The NanoPlotters employed for our protein profiling services:
• have CVs < 5% at 400 picoliter spotting volumes
• allow flexible array configuration for optimal study design
• are capable of printing a protein monolayer from crude sample lysates
Zeptosens sells a configuration of the NanoPlotterTM (trademark of GeSiM) that is adapted to ZeptoMARK reverse arrays. Standard reverse array configurations are:
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PN | Name | Channels | Chips / Arrays | Samples / Microplates | Comment | | 1900 | NanoPlotter 2.1 | 3 | 80 / 480 | 384 / 1 | for smaller studies | | 1920 | NanoPlotter 2.1/E | 3 | 123 / 738 | 2304 / 6 | for larger studies | | 1925 | NanoPlotter 2.1/E | 6 | 80 / 480 | 3132 / 9 | for screening applications |
All configurations include a humidifier for a controlled spotting environment and a cooling system for the chip and sample plate deck. See the NanoPlotter flyer for more information.
Zeptosens facilitates starting your in-house microarray system by providing all necessary buffers, chips, and protocols for immediate success with your reverse array experiments. Order information:
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