|
Zeptosens has developed an innovative, highly efficient and flexible solution for the analysis of cell signaling pathways. The ultra sensitive reverse protein microarray platform allows the investigation of compounds and their modulation of signaling pathway activities. Select from over 200 reverse array validated antibodies in Akt, MAPK, lipid, cAMP, insulin and many other signaling pathways or use your own western blot type antibodies which can easily be validated for use on ZeptoMARK reverse arrays.
ZeptoMARK Reverse Array Applications
Zeptosens provides services and reverse protein array systems for measurement of protein expression and post-translational modifications (phosphorylation, methylation, ...). Below is a selection of ZeptoMARK reverse array applications:
• Biomarker / Target Discovery • Biomarker / Target Validation
• Cell-Based Screening • Mode of Action Profiling • Selectivity Profiling
• Toxicology Investigation
Please contact
This email address is being protected from spam bots, you need Javascript enabled to view it
if your specific field or application is not listed or if you want to have more information about the ZeptoMARK reverse arrays.
Multiplexing with Reverse Arrays vs. Forward ArraysZeptoMARK Reverse Arrays
| ZeptoMARK Forward Arrays
 | | Lysed cells are immobilized on the chip. Antibodies are used to selectively detect protein expression and activation levels in the sample lysates. | Immobilized antibodies on the chip are used to selectively capture target proteins or small molecules in complex biological matrices. | | • just one western blot type detection antibody per target required | • two detection antibodies per target required | | • reverse array format allows for multiplexing of >1000 assays | • forward array format allows multiplexing of max. 15 assays
| | • fast validation, 99% of single band western blot antibodies work on reverse arrays | • as detection antibodies are mixed for measurement, the panel of antibodies is very limited and validation difficult and time-consuming | • put together the assays you want to measure with every new study without additional validation | • studies limited to the assay sets given
|
|
